GOLPH4 Antibody (PSH02-98)

Flow Cytometry: GOLPH4 Antibody (PSH02-98) [NBP3-32399]

Flow Cytometry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Flow cytometric analysis of HeLa cells labeling GOLPH4.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32399, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: GOLPH4 Antibody (PSH02-98) [NBP3-32399]

Western Blot: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Western blot analysis of GOLPH4 on different lysates with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: LO2 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: SiHa cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 82 kDa
Observed band size: 130/82 kDa

Exposure time:
Lane 1-6 (left): 1 minute 2 seconds;
Lane 1-6 (right): 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.