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GOT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00195

Novus Biologicals, part of Bio-Techne
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NBP1-00195

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SNLKKEGSTHNWQH corresponding to internal region according to NP_002071.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GOT2 Antibody

Western Blot: GOT2 Antibody [NBP1-00195]

Western Blot: GOT2 Antibody [NBP1-00195]

Western Blot: GOT2 Antibody [NBP1-00195] - Western blot analysis of GOT2 (aa 360 to 373) in Human Liver cell lysate (35ug protein in RIPA buffer) using NBP1-00195 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GOT2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human liver lysates (calculated MW of 47.5 kDa band according to NP_002071.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Entrez Gene IDs

2806 (Human); 14719 (Mouse); 25721 (Rat)

Gene Symbol

GOT2

Additional GOT2 Products

Product Documents for GOT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GOT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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