GP-IX Antibody (A5E6-R)

Western Blot: GP-IX Antibody (A5E6-R) [NBP3-32152]

Western Blot: GP-IX Antibody (A5E6-R) [NBP3-32152] - Western blot analysis of GP-IX on different lysates with Mouse anti-GP-IX antibody (NBP3-32152) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse lung tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 19 kDa
Observed band size: 28 kDa

Exposure time: 1 minute 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32152) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GP-IX Antibody (A5E6-R) [NBP3-32152]

Immunohistochemistry: GP-IX Antibody (A5E6-R) [NBP3-32152] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-GP-IX antibody (NBP3-32152) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32152) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.