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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # GP2/1712

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary) (Uniprot: P55259)

Reactivity Notes

Does not react with Mouse.

Localization

Cell Surface and Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54416)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GP2 Antibody (GP2/1712)

Western Blot: GP2 Antibody (GP2/1712) [NBP2-53322]

Western Blot: GP2 Antibody (GP2/1712) [NBP2-53322]

Western Blot: GP2 Antibody (GP2/1712) [NBP2-53322] - Western Blot Analysis of human Pancreas tissue lysate using GP2 antibody (GP2/1712).
Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) [NBP2-53322]

Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) [NBP2-53322]

Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) [NBP2-53322] - Formalin-fixed, paraffin-embedded human Pancreas stained with GP2 Monoclonal Antibody (GP2/1712).
Protein Array: GP2 Antibody (GP2/1712) [NBP2-53322]

Protein Array: GP2 Antibody (GP2/1712) [NBP2-53322]

Protein Array: GP2 Antibody (GP2/1712) [NBP2-53322] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GP2 Antibody (GP2/1712) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for GP2 Antibody (GP2/1712)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GP2/ZAP75

Long Name

Glycoprotein 2

Alternate Names

GP2, ZAP75

Gene Symbol

GP2

UniProt

Additional GP2/ZAP75 Products

Product Documents for GP2 Antibody (GP2/1712)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GP2 Antibody (GP2/1712)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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