gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04346

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H3

Format

BSA Free

Concentration

1.0 mg/ml

View all gp96/HSP90B1 Antibodies »

Product Specifications

Immunogen

Recombinant human gp96/HSP90B1/GRP94 (676-803aa) purified from E. coli

Marker

ER Stress Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3)BSA Free [NBP1-04346]

Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3)BSA Free [NBP1-04346]

Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]

Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]

Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)
Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]

Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]

Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

2-5ug- 1-106cells

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:100-1:200

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gp96/HSP90B1

Tumor rejection antigen 1(TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.

Long Name

Heat Shock Protein 90 beta 1

Alternate Names

ECGP, Endoplasmin, Grp94, HSP90B1

Gene Symbol

HSP90B1

UniProt

NP_003290 (Human)

Additional gp96/HSP90B1 Products

Product Documents for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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