GPR109B/HM74 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35830
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-392 of human GPR109B/HM74 (NP_006009.2).
Sequence:
SFPNFFSTLINRCLQRKITGEPDNNRSTSVELTGDPNKTRGAPEALIANSGEPWSPSYLGPTSNNHSKKGHCHQEPASLEKQLGCCIE
Sequence:
SFPNFFSTLINRCLQRKITGEPDNNRSTSVELTGDPNKTRGAPEALIANSGEPWSPSYLGPTSNNHSKKGHCHQEPASLEKQLGCCIE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GPR109B/HM74 Antibody
Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] -
Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] -
Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western Blot: GPR109B/HM74 Antibody [NBP3-35830] -
Western Blot: GPR109B/HM74 Antibody [NBP3-35830] - Western blot analysis of lysates from Rat pancreas, using GPR109B/HM74 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.
Applications for GPR109B/HM74 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GPR109B/HM74
Long Name
G Protein-coupled Receptor HM74 Protein-coupled Receptor 109B
Alternate Names
HCA3, HCAR3, HM74, NIACR2
Gene Symbol
HCAR3
Additional GPR109B/HM74 Products
Product Documents for GPR109B/HM74 Antibody
Product Specific Notices for GPR109B/HM74 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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