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GPR114 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87285

Novus Biologicals, part of Bio-Techne
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NBP1-87285
NBP1-87285-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETWEELLSYMENMQVSRGRSSVFSSRQLHQLEQMLLNTSFPGYNLTLQTPTIQSLAFKLSCDFSGLSLTSATLKRVPQAGGQHARGQHAMQFPAELTRDACKTRPRELRLICIYFSNTHFFKDENNSSLLNNYVLGAQLSHGHVNNLRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPR114 Antibody

Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285]

Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285]

Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285] - Staining in human lymph node and placenta tissues using anti-ADGRG5 antibody. Corresponding ADGRG5 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: GPR114 Antibody [NBP1-87285]

Immunocytochemistry/ Immunofluorescence: GPR114 Antibody [NBP1-87285]

Immunocytochemistry/Immunofluorescence: GPR114 Antibody [NBP1-87285] - Staining of human cell line CACO-2 shows localization to nucleoplasm, nuclear membrane & vesicles.
Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285]

Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285]

Immunohistochemistry-Paraffin: GPR114 Antibody [NBP1-87285] - Staining of human placenta shows low expression as expected.

Applications for GPR114 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR114

Orphan receptor

Long Name

G Protein-coupled Receptor 114

Alternate Names

PGR27

Gene Symbol

ADGRG5

Additional GPR114 Products

Product Documents for GPR114 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR114 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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