GPR50 Antibody

Western Blot: GPR50 Antibody [H00009248-B01P]

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 promotes ligand-independent activation of TBRI signaling. Detection of p-Smad3, p-Stat-3, p-Jak2 (c) and p-Smad2 (d) in lysates of hypothalamus and cortex of wt and GPR50 ko mice. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.

Western Blot: GPR50 Antibody [H00009248-B01P]

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TBRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.

Western Blot: GPR50 Antibody [H00009248-B01P] -

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T betaRI independently of T betaRII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T betaRI in lysates of SNU638 cells expressing HA-T betaRI alone or together with GPR50 delta4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T betaRI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T betaRIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T betaRI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GPR50 Antibody [H00009248-B01P] -

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T betaRI independently of T betaRII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T betaRI in lysates of SNU638 cells expressing HA-T betaRI alone or together with GPR50 delta4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T betaRI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T betaRIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T betaRI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.