GPT Antibody (4E2)
Novus Biologicals, part of Bio-Techne | Catalog # H00002875-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GPT (AAH18207.1, 1 a.a. ~ 496 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEELSRFHAKFTLEYS
Specificity
GPT - glutamic-pyruvate transaminase (alanine aminotransferase)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GPT Antibody (4E2)
![Western Blot: GPT Antibody (4E2) [H00002875-M03]](https://resources.bio-techne.com/images/products/GPT-Antibody-4E2-Western-Blot-H00002875-M03-img0003.jpg "Western Blot: GPT Antibody (4E2) [H00002875-M03]")
Western Blot: GPT Antibody (4E2) [H00002875-M03]
Western Blot: GPT Antibody (4E2) [H00002875-M03] - Analysis of GPT expression in transfected 293T cell line by GPT monoclonal antibody (M03), clone 4E2. Lane 1: GPT transfected lysatE (55 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: GPT Antibody (4E2) [H00002875-M03] - Detection limit for recombinant GST tagged FLJ23834 is approximately 1ng/ml as a capture antibody.
Applications for GPT Antibody (4E2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GPT
Alternate Names
AAT1alanine aminotransferase 1, ALT1, ALT1EC 2.6.1.2, Glutamate pyruvate transaminase 1, glutamic-alanine transaminase 1, Glutamic--alanine transaminase 1, glutamic-pyruvate transaminase (alanine aminotransferase), Glutamic--pyruvic transaminase 1, GPT1GPT 1
Entrez Gene IDs
2875 (Human)
Gene Symbol
GPT
OMIM
138200 (Human)
UniProt
Additional GPT Products
Product Documents for GPT Antibody (4E2)
Product Specific Notices for GPT Antibody (4E2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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