GPT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14072

Novus Biologicals, part of Bio-Techne
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NBP2-14072
NBP2-14072-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GPT2 Antibodies »

Product Summary for GPT2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPT2 Antibody

Western Blot: GPT2 Antibody [NBP2-14072]

Western Blot: GPT2 Antibody [NBP2-14072]

Western Blot: GPT2 Antibody [NBP2-14072] - Analysis in human cell lines Caco-2 and HeLa using anti-GPT2 antibody. Corresponding GPT2 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072]

Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072]

Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072]

Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072]

Immunohistochemistry-Paraffin: GPT2 Antibody [NBP2-14072] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
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Applications for GPT2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPT2

GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.[supplied by OMIM]

Alternate Names

AAT2, alanine aminotransferase 2, ALT2EC 2.6.1.2, Glutamate pyruvate transaminase 2, glutamic pyruvate transaminase (alanine aminotransferase) 2, Glutamic--alanine transaminase 2, glutamic-pyruvate transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

Gene Symbol

GPT2

Additional GPT2 Products

Product Documents for GPT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GPT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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