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Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80756

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 1A8

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Ly-6G transfected EL-4J cell line.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free

Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756]

Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756]

Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756] - Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer. This KO validation image was submitted via customer review. Image from the FITC version of this antibody.
Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756]

Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756]

Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free [NBP2-80756] - Detection of Ly-6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6G Monoclonal Antibody (Catalog # NBP2-53131, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113). Image from the standard format of this antibody.

Applications for Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.25 ug/10^6 cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6G/Ly-6C (Gr-1)

The mouse Gr-1 antigen is a GPI-linked myeloid differentiation marker also known as Ly-6G. Its cellular expression is restricted to monocytes within bone and on neutrophils in peripheral organs.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Gene Symbol

LY6G6D

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Gr-1/Ly-6G Antibody (1A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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