GR/NR3C1 Antibody (BuGR2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-731

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast

Cited:

Human, Mouse

Applications

Validated:

Block/Neutralize, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BuGR2

Format

BSA Free

Concentration

LYOPH mg/ml

View all GR/NR3C1 Antibodies »

Product Specifications

Immunogen

Partially purified rat GR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Specificity

Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GR/NR3C1 Antibody (BuGR2) - BSA Free

Western Blot: GR/NR3C1 Antibody (BuGR2)BSA Free [NB300-731]

Western Blot: GR/NR3C1 Antibody (BuGR2)BSA Free [NB300-731]

Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
View 6 more images

Applications for GR/NR3C1 Antibody (BuGR2) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1:10 - 1:1000

Gel Super Shift Assays

1:10 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

5 ug/mL

Immunoprecipitation

1:10 - 1:500

Western Blot

5 ug/mL
Application Notes
Blocking, ChIP, and ELISA usages were reported in scientific literature. Use in ICC/IF was reported in scientific literature (PMID: 30402116)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3 using NB300-731 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 0.1 ml sterilized water to desired concentration.

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Entrez Gene IDs

2908 (Human); 14815 (Mouse); 24413 (Rat)

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (BuGR2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GR/NR3C1 Antibody (BuGR2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.