GR/NR3C1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42221

Novus Biologicals, part of Bio-Techne
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NBP2-42221
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Bovine

Predicted:

Feline (93%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation Sequencing, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Chemotaxis, Chip Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GR/NR3C1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GR/NR3C1. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (Ruiz-Conca M et al). Mouse (89%), Rat (87%), Sheep (88%), Canine (87%), Guniea Pig (87%), Porcine (83%).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GR/NR3C1 Antibody

Knockout Validated: GR/NR3C1 Antibody [NBP2-42221]

Knockout Validated: GR/NR3C1 Antibody [NBP2-42221]

Knockout Validated: GR/NR3C1 Antibody [NBP2-42221] - Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: GR/NR3C1 Antibody [NBP2-42221]

Western Blot: GR/NR3C1 Antibody [NBP2-42221]

Western Blot: GR/NR3C1 Antibody [NBP2-42221] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human lung cancer. Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GR/NR3C1)
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Applications for GR/NR3C1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

Reported in scientific literature (PMID:35077705)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GR/NR3C1

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Gene Symbol

NR3C1

UniProt

P04150

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GR/NR3C1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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