GRAF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89732

Novus Biologicals, part of Bio-Techne
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NBP1-89732
NBP1-89732-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRAF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSNPNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%). Reactivity reported in scientific literature (PMID: 23319410)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAF Antibody

Western Blot: GRAF Antibody [NBP1-89732]

Western Blot: GRAF Antibody [NBP1-89732]

Western Blot: GRAF Antibody [NBP1-89732] - Analysis in human cell lines Caco-2 and MCF-7 using anti-ARHGAP26 antibody. Corresponding ARHGAP26 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: GRAF Antibody [NBP1-89732]

Immunocytochemistry/ Immunofluorescence: GRAF Antibody [NBP1-89732]

Immunocytochemistry/Immunofluorescence: GRAF Antibody [NBP1-89732] - Jurkat T cells expressing GFP-Cdc42-Q61L, activated, fixed and stained with an antibody against endogenous GRAF. Arrows indicate GRAF-positive tubules. Images representative of at least three independent experiments. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31690048/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: GRAF Antibody [NBP1-89732]

Immunohistochemistry-Paraffin: GRAF Antibody [NBP1-89732]

Immunohistochemistry-Paraffin: GRAF Antibody [NBP1-89732] - Staining in human cerebral cortex and pancreas tissues using anti-ARHGAP26 antibody. Corresponding ARHGAP26 RNA-seq data are presented for the same tissues.
View 2 more images

Applications for GRAF Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31690048).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRAF

GTPase regulator associated with the focal adhesion kinase pp125.

Long Name

Rho GTPase Activating Protein 26

Alternate Names

ARHGAP26, OPHN1L1

Entrez Gene IDs

23092 (Human)

Gene Symbol

ARHGAP26

UniProt

Q9UNA1 (Human)

Additional GRAF Products

Product Documents for GRAF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRAF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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