Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all GRAMD1A Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 674 to 724 of human GRAM Domain Containing 1A using the numbering given in entry NP_065946.2 (GeneID 57655).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAMD1A Antibody - BSA Free

Western Blot: GRAMD1A Antibody [NBP2-32148]

Western Blot: GRAMD1A Antibody [NBP2-32148]

Western Blot: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, MCF7, Jurkat, and K562 cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: GRAMD1A Antibody [NBP2-32148]

Immunoprecipitation: GRAMD1A Antibody [NBP2-32148]

Immunoprecipitation: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated GRAMD1A, NBP2-32148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GRAMD1A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

6 µg/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GRAMD1A

Alternate Names

FLJ22411, FLJ90346, GRAM domain containing 1A, GRAM domain-containing protein 1A, KIAA1533

Gene Symbol

GRAMD1A

UniProt

NP_065946.2 (Human)

Additional GRAMD1A Products

Product Documents for GRAMD1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GRAMD1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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