Grancalcin Antibody (2F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00025801-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Grancalcin Antibodies »

Product Specifications

Immunogen

GCA (AAH05214, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

Reactivity Notes

Human. Other species not tested.

Specificity

GCA (2F5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Grancalcin Antibody (2F5)

Western Blot: Grancalcin Antibody (2F5) [H00025801-M01]

Western Blot: Grancalcin Antibody (2F5) [H00025801-M01]

Western Blot: Grancalcin Antibody (2F5) [H00025801-M01] - GCA monoclonal antibody (M01), clone 2F5 Analysis of GCA expression in MCF-7.
Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01]

Immunocytochemistry/ Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01]

Immunocytochemistry/Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01] - Analysis of monoclonal antibody to GCA on MCF-7 cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Grancalcin Antibody (2F5) [H00025801-M01] - Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.

Applications for Grancalcin Antibody (2F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Grancalcin

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Alternate Names

EF-hand calcium-binding protein, grancalcin, EF-hand calcium binding protein, grancalcin, penta-EF-hand protein

Entrez Gene IDs

25801 (Human)

Gene Symbol

GCA

OMIM

607030 (Human)

UniProt

AAH05214 (Human)

Additional Grancalcin Products

Product Documents for Grancalcin Antibody (2F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Grancalcin Antibody (2F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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