Granulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87324

Novus Biologicals, part of Bio-Techne
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NBP1-87324
NBP1-87324-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Granulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLL

Reactivity Notes

Mouse reactivity reported in (PMID: 29511098). Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granulin Antibody

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues . Corresponding GRN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Granulin Antibody [NBP1-87324]

Immunocytochemistry/ Immunofluorescence: Granulin Antibody [NBP1-87324]

Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87324] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324]

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
View 3 more images

Applications for Granulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reported in scientific literature (PMID: 29511098)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granulin

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Alternate Names

acrogranin, GEP, GP88, granulin, granulin-epithelin, granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, progranulin

Gene Symbol

GRN

Additional Granulin Products

Product Documents for Granulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Granulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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