Granzyme B Antibody (BLR022E)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76414

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76414
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR022E

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Granzyme B Antibodies »

Product Specifications

Immunogen

Recombinant protein representing Human Granzyme B

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granzyme B Antibody (BLR022E)

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414]

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414]

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody.
Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of Granzyme B over-expressing cells (left) and Granzyme H overexpressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.
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Applications for Granzyme B Antibody (BLR022E)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence 1:250

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Granzyme B

Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

Additional Granzyme B Products

Product Documents for Granzyme B Antibody (BLR022E)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Granzyme B Antibody (BLR022E)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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