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Granzyme B Antibody (GrB-7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51746

Novus Biologicals, part of Bio-Techne
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NBP1-51746

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GrB-7

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

The antibody reacts with the 33 kD human serine protease granzyme B.

Specificity

Does not react with human granzyme A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Granzyme B Antibody (GrB-7)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
The monoclonal antibody stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. It does not react with other cell types. The antibody can not be used on frozen sections. The antibBefore use in immunocytochemistry the slides have to be pretreated with 0.1 M sodium citrate, for 10 min. at 100 degrees C (in microwave oven). A working dilution of 1:20 is advised but optimal dilution should be tested by serial dilution.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Serum free tissue culture supernatant, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Granzyme B

Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

Additional Granzyme B Products

Product Documents for Granzyme B Antibody (GrB-7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Granzyme B Antibody (GrB-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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