Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E4

Concentration

1 mg/ml

View all Granzyme B Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 21-247 of human GZMB(NP_004122) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Granzyme B Antibody (OTI4E4)

Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.
Immunohistochemistry: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Immunohistochemistry: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Immunohistochemistry: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732]

Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of extracts (10ug) from 3 different cell lines.
View 2 more images

Applications for Granzyme B Antibody (OTI4E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

Additional Granzyme B Products

Product Documents for Granzyme B Antibody (OTI4E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Granzyme B Antibody (OTI4E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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