Granzyme H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86565

Novus Biologicals, part of Bio-Techne
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NBP1-86565
NBP1-86565-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Granzyme H Antibodies »

Product Summary for Granzyme H Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQVKPGQLCSVAGWGYVSMSTLATTLQEVLLTVQKDCQCERLFHGNYSRATEICVGDPKKTQTGFKGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Granzyme H Antibody

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565] - Staining in human spleen and pancreas tissues using anti-GZMH antibody. Corresponding GZMH RNA-seq data are presented for the same tissues.
Western Blot: Granzyme H Antibody [NBP1-86565]

Western Blot: Granzyme H Antibody [NBP1-86565]

Western Blot: Granzyme H Antibody [NBP1-86565] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565] - Staining of human pancreas shows low expression as expected.
View 1 more images

Applications for Granzyme H Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granzyme H

The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses.

Alternate Names

CCP-X, CGL2, CTSGL2, GrzH, GZMH

Entrez Gene IDs

2999 (Human)

Gene Symbol

GZMH

UniProt

P20718

Additional Granzyme H Products

Product Documents for Granzyme H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Granzyme H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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