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GRASP65 Antibody (3F2I1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16113

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16113-100ul
NBP3-16113-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3F2I1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 341-440 of human GRASP65 (Q9BQQ3). SGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRASP65 Antibody (3F2I1)

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Immunofluorescence analysis of U-2 OS cells using GRASP65 Rabbit mAb (NBP3-16113) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]

Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for GRASP65 Antibody (3F2I1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRASP65

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Alternate Names

Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa

Gene Symbol

GORASP1

Additional GRASP65 Products

Product Documents for GRASP65 Antibody (3F2I1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRASP65 Antibody (3F2I1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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