GRASP65 Antibody (3F2I1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16113
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3F2I1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 341-440 of human GRASP65 (Q9BQQ3). SGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GRASP65 Antibody (3F2I1)
Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]
Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113]
Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Immunofluorescence analysis of U-2 OS cells using GRASP65 Rabbit mAb (NBP3-16113) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113]
Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for GRASP65 Antibody (3F2I1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GRASP65
Alternate Names
Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa
Gene Symbol
GORASP1
Additional GRASP65 Products
Product Documents for GRASP65 Antibody (3F2I1)
Product Specific Notices for GRASP65 Antibody (3F2I1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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