GRHL2 Antibody (CL3760)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61634

Novus Biologicals, part of Bio-Techne
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NBP2-61634
NBP2-61634-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3760

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRHL2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NRVQVLKTVPVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSSGTFQYTLEATKSLRQK

Epitope

VVKAEDFTPV

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (87%), Rat (87%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRHL2 Antibody (CL3760)

Immunocytochemistry/ Immunofluorescence: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunocytochemistry/ Immunofluorescence: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunocytochemistry/Immunofluorescence: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of A-431 cells using the Anti-GRHL2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634]

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human rectum shows strong nuclear immunoreactivity in glandular cells.
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Applications for GRHL2 Antibody (CL3760)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRHL2

TFCP2L3 is a member of a family of transcription factor genes whose archetype is TFCP2 (MIM 189889), a mammalian ortholog of the Drosophila gene 'grainyhead' (grh).[supplied by OMIM]

Alternate Names

BOMMGC149294, Brother of mammalian grainyhead, deafness, autosomal dominant 28, DFNA28, FLJ13782, grainyhead-like 2 (Drosophila), grainyhead-like protein 2 homolog, TFCP2L3MGC149295, Transcription factor CP2-like 3FLJ11172

Gene Symbol

GRHL2

Additional GRHL2 Products

Product Documents for GRHL2 Antibody (CL3760)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRHL2 Antibody (CL3760)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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