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GRIM19 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90050

Novus Biologicals, part of Bio-Techne
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NBP1-90050
NBP1-90050-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IMKWNRERRRLQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPPLIGELYGLRTTEEALHASHGFMWYT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRIM19 Antibody

Western Blot: GRIM19 Antibody [NBP1-90050]

Western Blot: GRIM19 Antibody [NBP1-90050]

Western Blot: GRIM19 Antibody [NBP1-90050] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: GRIM19 Antibody [NBP1-90050]

Immunocytochemistry/ Immunofluorescence: GRIM19 Antibody [NBP1-90050]

Immunocytochemistry/Immunofluorescence: GRIM19 Antibody [NBP1-90050] - Staining of human cell line U-251 MG shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP1-90050]

Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP1-90050]

Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP1-90050] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for GRIM19 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRIM19

The GRIM19 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transf

Alternate Names

B16.6, CDA016, Cell death regulatory protein GRIM-19, cell death-regulatory protein GRIM19, CI-B16.6, complex I B16.6 subunit, Complex I-B16.6, FLJ58045, FLJ59191, Gene associated with retinoic and IFN-induced mortality 19 protein, Gene associated with retinoic and interferon-induced mortality 19 protein, GRIM-19CGI-39, GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Gene Symbol

NDUFA13

Additional GRIM19 Products

Product Documents for GRIM19 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRIM19 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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