GRIM19 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human GRIM19 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for GRIM19 Antibody
Western Blot: GRIM19 Antibody [NBP2-98488]
Western Blot: GRIM19 Antibody [NBP2-98488] - Anti-GRIM19 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP2-98488]
Immunohistochemistry-Paraffin: GRIM19 Antibody [NBP2-98488] - Immunochemical staining of human GRIM19 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Immunoprecipitation: GRIM19 Antibody [NBP2-98488]
Immunoprecipitation: GRIM19 Antibody [NBP2-98488] - GRIM19 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 4 ul anti-GRIM19 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-GRIM19 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDaApplications for GRIM19 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRIM19
Alternate Names
B16.6, CDA016, Cell death regulatory protein GRIM-19, cell death-regulatory protein GRIM19, CI-B16.6, complex I B16.6 subunit, Complex I-B16.6, FLJ58045, FLJ59191, Gene associated with retinoic and IFN-induced mortality 19 protein, Gene associated with retinoic and interferon-induced mortality 19 protein, GRIM-19CGI-39, GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
Gene Symbol
NDUFA13
Additional GRIM19 Products
Product Specific Notices for GRIM19 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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