Key Product Details

Species Reactivity

Human, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D11

Format

BSA Free

Concentration

1 mg/ml

View all GRK1 Antibodies »

Product Specifications

Immunogen

Full length human GRK1.

Epitope

This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Specificity

Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This does not cross-react with other G protein-coupled kinases.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRK1 Antibody (D11) - BSA Free

Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (D11) [NB120-2776] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (D11) [NB120-2776] - Immunolocalization of GRK1a/b in bovine retina
Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/ Immunofluorescence: GRK1 Antibody (D11) - BSA Free [NB120-2776]

Immunocytochemistry/Immunofluorescence: GRK1 Antibody (D11) [NB120-2776] - Analysis of Rhodopsin Kinase 1a/b (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
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Applications for GRK1 Antibody (D11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000
Application Notes
WB: Detects an approx. 60 kDa protein representing GRK 1a/b. ab2766 detects both in vitro expressed splice variants by WB but was not able to detect significant amounts of GRK1b from human retina. IF: Staining of GRK1a/b in bovine retina with ab2766 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRK1

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Long Name

G protein-coupled Receptor Kinase 1

Alternate Names

GPRK1, RHOK

Entrez Gene IDs

6011 (Human)

Gene Symbol

GRK1

Additional GRK1 Products

Product Documents for GRK1 Antibody (D11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRK1 Antibody (D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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