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GRK2 Antibody (3F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37611

Novus Biologicals, part of Bio-Techne
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NBP2-37611
NBP2-37611-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human GRK2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRK2 Antibody (3F8) - BSA Free

Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute and recovery phases. Micrographs showing immunofluorescence stainings of GRK2 in the acute and recovery phases. Arrowheads (yellow) indicate positive signals for GRK2. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.
Western Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]

Western Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]

Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Applications for GRK2 Antibody (3F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for GRK2 Antibody (3F8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRK2 Antibody (3F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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