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GRK2 [p Ser670] Antibody (HL1035)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13688

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-13688

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1035

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser670 of human GRK2. The exact sequence is proprietary.

Modification

p Ser670

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GRK2 [p Ser670] Antibody (HL1035)

Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] -

Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for GRK2 [p Ser670] Antibody (HL1035)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for GRK2 [p Ser670] Antibody (HL1035)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRK2 [p Ser670] Antibody (HL1035)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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