GRK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2831

Novus Biologicals, part of Bio-Techne
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NB100-2831
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GRK2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RDAYREAQQLVQR corresponding to internal region according to NP_001610.2.

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GRK2 Antibody

Western Blot: GRK2 Antibody [NB100-2831]

Western Blot: GRK2 Antibody [NB100-2831]

Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.

Applications for GRK2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRK2

Beta-adrenergic receptor kinase (ADRBK1) phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. The ADRBK1 gene spans approximately 23 kb and is composed of 21 exons. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. Inhibition of ADRBK1 is a novel mode of therapy.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Entrez Gene IDs

156 (Human); 110355 (Mouse)

Gene Symbol

GRK2

UniProt

NP_001610.1 (Human)

Additional GRK2 Products

Product Documents for GRK2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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