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GRK6 Antibody (JE63-06)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32406

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32406

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE63-06

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human GRK6 aa 71-120 / 576. (Uniprot: P43250)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRK6 Antibody (JE63-06)

GRK6 Antibody (JE63-06)

Western Blot: GRK6 Antibody (JE63-06) [NBP3-32406]

Western Blot: GRK6 Antibody (JE63-06) [NBP3-32406] - Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (NBP3-32406) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate
Lane 6: A549 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32406) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
GRK6 Antibody (JE63-06)

Flow Cytometry: GRK6 Antibody (JE63-06) [NBP3-32406]

Flow Cytometry: GRK6 Antibody (JE63-06) [NBP3-32406] - Flow cytometric analysis of 293T cells labeling GRK6.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32406, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications for GRK6 Antibody (JE63-06)

Application
Recommended Usage

Flow Cytometry

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRK6

GRK6 encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.

Alternate Names

EC 2.7.11, EC 2.7.11.16, FLJ32135, G protein-coupled receptor kinase 6, GPRK6G protein-coupled receptor kinase GRK6

Gene Symbol

GRK6

Additional GRK6 Products

Product Documents for GRK6 Antibody (JE63-06)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRK6 Antibody (JE63-06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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