Growth Hormone Antibody (5E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26555

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Growth Hormone Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Growth Hormone [UniProt P01241]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Growth Hormone Antibody (5E9)

Growth Hormone Antibody (5E9)

Immunohistochemistry: Growth Hormone Antibody (5E9) [NBP3-26555] -

Immunohistochemistry: Growth Hormone Antibody (5E9) [NBP3-26555] - Image of Growth Hormone Antibody (5E9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Growth Hormone Antibody (5E9)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Growth Hormone

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Gene Symbol

GH1

Additional Growth Hormone Products

Product Documents for Growth Hormone Antibody (5E9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Growth Hormone Antibody (5E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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