Growth Hormone Antibody (GH-45) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-364

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GH-45

Format

BSA Free

Concentration

0.5 mg/ml

View all Growth Hormone Antibodies »

Product Specifications

Immunogen

Human growth hormone

Specificity

The antibody GH-45 reacts with human growth hormone (hGH), a polypeptide hormone synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. The GH-45 antibody reacts with affinity constant 3.8 x 1010 l /mol; it does not bind human prolactin or any other pituitary hormones.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Growth Hormone Antibody (GH-45) - BSA Free

ELISA: Growth Hormone Antibody (GH-45) - BSA Free [NB500-364]

ELISA: Growth Hormone Antibody (GH-45) - BSA Free [NB500-364]

ELISA: Growth Hormone Antibody (GH-45) [NB500-364] - ELISA of human growth hormone using mouse monoclonal antibody GH-45. Compared with mouse IgG1 isotype control antibody MOPC-21.

Applications for Growth Hormone Antibody (GH-45) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

1:200
Application Notes
ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Growth Hormone

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression. Detection of growth hormone producing cells

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

Additional Growth Hormone Products

Product Documents for Growth Hormone Antibody (GH-45) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Growth Hormone Antibody (GH-45) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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