Growth Hormone Antibody (SPM106) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54352
![Immunohistochemistry-Paraffin: Growth Hormone Antibody (SPM106) - Azide and BSA Free [NBP2-54352]](https://resources.bio-techne.com/images/products/Growth-Hormone-Antibody-SPM106-Immunohistochemistry-Paraffin-NBP2-54352-img0001.jpg "Immunohistochemistry-Paraffin: Growth Hormone Antibody (SPM106) - Azide and BSA Free [NBP2-54352]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM106
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A recombinant fragment (around aa58-187) of human Growth Hormone (GH) protein (exact sequence is proprietary) (Uniprot: P01241)
Localization
Cytoplasmic
Specificity
Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).
Marker
Pituitary Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53263).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Growth Hormone Antibody (SPM106) - Azide and BSA Free
Immunohistochemistry-Paraffin: Growth Hormone Antibody (SPM106) - Azide and BSA Free [NBP2-54352]
Immunohistochemistry-Paraffin: Growth Hormone Antibody (SPM106) [NBP2-54352] - Formalin-fixed, paraffin-embedded Human Pituitary stained with Growth Hormone Monoclonal Antibody (SPM106).Applications for Growth Hormone Antibody (SPM106) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Growth Hormone
Alternate Names
GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin
Gene Symbol
GH1
Additional Growth Hormone Products
Product Documents for Growth Hormone Antibody (SPM106) - Azide and BSA Free
Product Specific Notices for Growth Hormone Antibody (SPM106) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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