GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47801

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI9F8

Concentration

1 mg/ml

View all GRP75/HSPA9B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein.

Marker

Immortalization Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

73.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) [NBP1-47801]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
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Applications for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Entrez Gene IDs

3313 (Human)

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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