GRP75/HSPA9B/Mortalin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31596

Novus Biologicals, part of Bio-Techne
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NBP1-31596
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Xenopus (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRP75/HSPA9B Antibodies »

Product Summary for GRP75/HSPA9B/Mortalin Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP75/HSPA9B/Mortalin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%).

Localization

Mitochondrion

Marker

Immortalization Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for GRP75/HSPA9B/Mortalin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

Suitable for use in Western Blot. Other applications not tested. Human and mouse Grp 75

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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