Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C5-1A4

Concentration

1 mg/ml

View all GRP78/HSPA5 Antibodies »

Product Specifications

Immunogen

Full-length recombinant rat GRP78

Localization

Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome

Specificity

Detects approx 33 kDa protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP78/HSPA5 Antibody (3C5-1A4)

Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692]

Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692]

Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692]

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692]

Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Applications for GRP78/HSPA5 Antibody (3C5-1A4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/mL of this antibody was sufficient for detection of GRP78 in 20 ug of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-59692 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3).In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody (3C5-1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRP78/HSPA5 Antibody (3C5-1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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