GRP78/HSPA5 Antibody (6H4-2G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12889
![Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]](https://resources.bio-techne.com/images/products/GRP78-HSPA5-Antibody-6H4-2G7-Western-Blot-NBP2-12889-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6H4-2G7
Concentration
1 mg/ml
Product Specifications
Immunogen
His-tagged human GRP78
Localization
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
Specificity
Detects a protein with a mass of approx. 78kDa corresponding to GRP78
Marker
ER Stress Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for GRP78/HSPA5 Antibody (6H4-2G7)
Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]
Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]](https://resources.bio-techne.com/images/products/GRP78-HSPA5-Antibody-6H4-2G7-Immunocytochemistry-Immunofluorescence-NBP2-12889-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]")
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.Applications for GRP78/HSPA5 Antibody (6H4-2G7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Application Notes
0.5 ug/ml of GRP78 Antibody was sufficient for detection of Grp78 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRP78/HSPA5
Long Name
75 KD Glucose Regulated Protein
Alternate Names
BIP, HSP70-5, HSPA5
Gene Symbol
HSPA5
Additional GRP78/HSPA5 Products
Product Documents for GRP78/HSPA5 Antibody (6H4-2G7)
Product Specific Notices for GRP78/HSPA5 Antibody (6H4-2G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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