GRP78/HSPA5 Antibody (8J5M2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16511

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16511
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8J5M2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRP78/HSPA5 Antibodies »

Product Summary for GRP78/HSPA5 Antibody (8J5M2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GRP78/HSPA5 (NP_005338.1). MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRP78/HSPA5 Antibody (8J5M2)

Western Blot: GRP78/HSPA5 Antibody (8J5M2) [NBP3-16511]

Western Blot: GRP78/HSPA5 Antibody (8J5M2) [NBP3-16511]

Western Blot: GRP78/HSPA5 Antibody (8J5M2) [NBP3-16511] - Western blot analysis of extracts of various cell lines, using GRP78/HSPA5 Rabbit mAb (NBP3-16511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for GRP78/HSPA5 Antibody (8J5M2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody (8J5M2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody (8J5M2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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