GRP78/HSPA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54318

Novus Biologicals, part of Bio-Techne
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NBP1-54318
NBP1-54318-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Drosophila, Hamster

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all GRP78/HSPA5 Antibodies »

Product Specifications

Immunogen

Full length human GRP78 (Bip) his tagged at the N terminus

Localization

Endoplasmic Reticulum , Endoplasmic Reticulum Membrane , Melanosome

Specificity

Detects a protein with a mass of approx. 78kDa corresponding to Grp78.

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRP78/HSPA5 Antibody

Western Blot: GRP78/HSPA5 Antibody [NBP1-54318]

Western Blot: GRP78/HSPA5 Antibody [NBP1-54318]

Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318]

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318]

Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.

Applications for GRP78/HSPA5 Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of GRP78 (Bip) Antibody was sufficient for detection of Grp78 in 10 ug of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

GRP78 is a ubiquitously expressed, 78-kDa glucoseregulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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