GRP78/HSPA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16749

Novus Biologicals, part of Bio-Techne
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NBP2-16749
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human

Predicted:

Bovine (100%), Fish (95%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRP78/HSPA5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP78/HSPA5. The exact sequence is proprietary.

Reactivity Notes

Japanese Medaka (95%), Xenopus laevis (99%).

Localization

Endoplasmic reticulum lumen; Melanosome

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP78/HSPA5 Antibody

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749]

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749]

Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749]

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749]

Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat duodenum. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 11 more images

Applications for GRP78/HSPA5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

UniProt

P11021 (Human)

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRP78/HSPA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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