GRSF1 Antibody (JE65-34)

Western Blot: GRSF1 Antibody (JE65-34) [NBP3-32410]

Western Blot: GRSF1 Antibody (JE65-34) [NBP3-32410] - Western blot analysis of GRSF1 on different lysates with Rabbit anti-GRSF1 antibody (NBP3-32410) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32410) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: GRSF1 Antibody (JE65-34) [NBP3-32410]

Immunocytochemistry/ Immunofluorescence: GRSF1 Antibody (JE65-34) [NBP3-32410] - Immunocytochemistry analysis of Hela cells labeling GRSF1 with Rabbit anti-GRSF1 antibody (NBP3-32410) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRSF1 antibody (NBP3-32410) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.