GSDMDC1 Antibody (8K8C6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33450
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 8K8C6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMDC1.3)
Sequence:
GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Sequence:
GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GSDMDC1 Antibody (8K8C6)
Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] -
Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] -
Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Western Blot: GSDMDC1 Antibody (8K8C6) [GSDMDC1] -
Western Blot: GSDMDC1 Antibody (8K8C6) [GSDMDC1] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Applications for GSDMDC1 Antibody (8K8C6)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:1000 - 1:3000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GSDMDC1
Long Name
Gasdermin-D
Alternate Names
DFNA5L, FKSG10, GSDMD, hGSDMD-CTD)
Gene Symbol
GSDMD
Additional GSDMDC1 Products
Product Documents for GSDMDC1 Antibody (8K8C6)
Product Specific Notices for GSDMDC1 Antibody (8K8C6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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