GSK-3 alpha/beta Antibody (1H8)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85523

Novus Biologicals, part of Bio-Techne
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NB110-85523
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Hamster, Primate, Rabbit, Sheep

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1H8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha/beta Antibodies »

Product Summary for GSK-3 alpha/beta Antibody (1H8)

Immunogen

Recombinant Xenopus GSK3 alpha/beta (1H8)

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

GSK3 alpha/beta Antibody (1H8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GSK-3 alpha/beta Antibody (1H8)

Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523]

Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523]

Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523] - Analysis: Lane 1: MW marker, Lane 2: 20ug HeLa lysate (heat shocked), Lane 3: 20ug mouse brain lysate, Lane 4: 20ug rat brain lysate, Lane 5: 20ug RK-13 lysate.
Immunohistochemistry-Paraffin: GSK-3 alpha/beta Antibody (1H8) [NB110-85523]

Immunohistochemistry-Paraffin: GSK-3 alpha/beta Antibody (1H8) [NB110-85523]

Immunohistochemistry-Paraffin: GSK3 alpha/beta Antibody (1H8) [NB110-85523] - Alzgeuner diseased neurons with granulovacuolar degeneration co-stained with GSK alpha/beta Monoclonal Antibody (1H8) (Brown Precipitate).

Applications for GSK-3 alpha/beta Antibody (1H8)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Western Blot, Immunohistochemistry-Paraffin. May be useful in Immunocytochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Entrez Gene IDs

50686 (Rat)

Gene Symbol

GSK3A

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta Antibody (1H8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSK-3 alpha/beta Antibody (1H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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