GSK-3 alpha/beta [p Ser9] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-741

Novus Biologicals, part of Bio-Techne
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NB300-741
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha/beta Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding GSK-3 alpha/beta conjugated to KLH. Accession # P18266

Modification

p Ser9

Specificity

Specific for the ~46 kDa GSK3 beta protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3beta band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 alpha/beta [p Ser9] Antibody

Western Blot: GSK-3 alpha/beta [p Ser9] Antibody [NB300-741]

Western Blot: GSK-3 alpha/beta [p Ser9] Antibody [NB300-741]

Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Applications for GSK-3 alpha/beta [p Ser9] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3a and GSK3b. Specifically, GSK3b has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3b by insulin (Sutherland et al., 1993).

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Entrez Gene IDs

2931 (Human); 606496 (Mouse); 50686 (Rat)

Gene Symbol

GSK3A

UniProt

P18266 (Rat)

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta [p Ser9] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSK-3 alpha/beta [p Ser9] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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