GSPT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91971

Novus Biologicals, part of Bio-Techne
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NBP1-91971
NBP1-91971-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GSPT2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSPT2 Antibody

Western Blot: GSPT2 Antibody [NBP1-91971]

Western Blot: GSPT2 Antibody [NBP1-91971]

Western Blot: GSPT2 Antibody [NBP1-91971] - Analysis in human cell line PC-3 and human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: GSPT2 Antibody [NBP1-91971]

Immunocytochemistry/ Immunofluorescence: GSPT2 Antibody [NBP1-91971]

Immunocytochemistry/Immunofluorescence: GSPT2 Antibody [NBP1-91971] - Staining of human cell line PC-3 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GSPT2 Antibody [NBP1-91971]

Immunohistochemistry-Paraffin: GSPT2 Antibody [NBP1-91971]

Immunohistochemistry-Paraffin: GSPT2 Antibody [NBP1-91971] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Applications for GSPT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSPT2

GSPT2 is closely related to GSPT1 (MIM 139259), a GTP-binding protein that plays an essential role at the G1- to S-phase transition of the cell cycle in yeast and human cells. GSPT1 is a positive regulator of translational accuracy and, in a binary complex with eRF1 (MIM 600285), functions as a polypeptide chain release factor.[supplied by OMIM]

Alternate Names

eRF3b, ERF3BGST2, eukaryotic peptide chain release factor GTP-binding subunit ERF3B, Eukaryotic peptide chain release factor subunit 3b, FLJ10441, G1 to S phase transition 2, G1 to S phase transition protein 2 homolog

Gene Symbol

GSPT2

Additional GSPT2 Products

Product Documents for GSPT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSPT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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