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GST Epitope Tag Antibody (6I7V6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16888

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16888

Key Product Details

Species Reactivity

Validated:

Epitope Tag

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6I7V6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for GST Epitope Tag Antibody (6I7V6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of schja GST protein (P08515). MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GST Epitope Tag Antibody (6I7V6)

Western Blot: GST Epitope Tag Antibody (6I7V6) [NBP3-16888]

Western Blot: GST Epitope Tag Antibody (6I7V6) [NBP3-16888]

Western Blot: GST Epitope Tag Antibody (6I7V6) [NBP3-16888] - Western blot analysis of extracts of GST protein, using GST-Tag antibody (NBP3-16888) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunoprecipitation: GST Epitope Tag Antibody (6I7V6) [NBP3-16888]

Immunoprecipitation: GST Epitope Tag Antibody (6I7V6) [NBP3-16888]

Immunoprecipitation: GST Epitope Tag Antibody (6I7V6) [NBP3-16888] - Immunoprecipitation analysis of 1ug extracts of GST-Tag cells using 3ug GST Epitope Tag antibody (NBP3-16888). Western blot was performed from the immunoprecipitate using GST Epitope Tag antibody (NBP3-16888) at a dilition of 1:1000.

Applications for GST Epitope Tag Antibody (6I7V6)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:2000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GST Epitope Tag

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody (6I7V6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GST Epitope Tag Antibody (6I7V6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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