Skip to main content

Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunohistochemistry, Knockout Validated

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Rabbits were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.

Reactivity Notes

Rat reactivity in vitro transcription/translation systems reported in scientific literature (PMID:19412421). Human reactivity reported in in vitro transcription/translation systems scientific literature (PMID: 18614808). Bacteria reported in (PMID: 27470678).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GST Epitope Tag Antibody [Biotin]

Western Blot: GST Epitope Tag Antibody [Biotin] [NB600-326B] - 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 60 seconds.

Applications for GST Epitope Tag Antibody [Biotin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GST Epitope Tag

GST (glutathione S-transferase) is commonly engineered onto the N- or C- terminus of a protein of interest to create fusion proteins that can be purified, visualized and analyzed via the properties of the GST tag.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GST Epitope Tag Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...