GSTA4 Antibody (JE61-65)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32412
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE61-65
Concentration
1 mg/ml
Product Summary for GSTA4 Antibody (JE61-65)
Immunogen
Synthetic peptide within Human GSTA4 aa 1-100. (Uniprot: O15217)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GSTA4 Antibody (JE61-65)
Western Blot: GSTA4 Antibody (JE61-65) [NBP3-32412]
Western Blot: GSTA4 Antibody (JE61-65) [NBP3-32412] - Western blot analysis of GSTA4 on different lysates with Rabbit anti-GSTA4 antibody (NBP3-32412) at 1/1,000 dilution.Lane 1: HEK-293 cell lysate (40 ug/Lane)
Lane 2: JAR cell lysate (40 ug/Lane)
Lane 3: Human liver tissue lysate (40 ug/Lane)
Lane 4: Human brain tissue lysate (30 ug/Lane)
Lane 5: Human kidney tissue lysate (30 ug/Lane)
Predicted band size: 26 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32412) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for GSTA4 Antibody (JE61-65)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GSTA4
Alternate Names
DKFZp686D21185, EC 2.5.1.18, glutathione S-alkyltransferase A4, glutathione S-aralkyltransferase A4, glutathione S-aryltransferase A4, glutathione S-transferase A4, Glutathione S-transferase A4-4, glutathione S-transferase alpha 4, glutathione transferase A4-4, GST class-alpha member 4, GSTA4-4, GTA4, S-(hydroxyalkyl)glutathione lyase A4
Gene Symbol
GSTA4
Additional GSTA4 Products
Product Documents for GSTA4 Antibody (JE61-65)
Product Specific Notices for GSTA4 Antibody (JE61-65)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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