GSTO1 Antibody (2C7)

Western Blot: GSTO1 Antibody (2C7) [NBP3-15189]

Western Blot: GSTO1 Antibody (2C7) [NBP3-15189] - Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: GSTO1 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 24, 25 KDa. Observed band size: 28 KDa.

Immunocytochemistry/ Immunofluorescence: GSTO1 Antibody (2C7) [NBP3-15189]

Immunocytochemistry/Immunofluorescence: GSTO1 Antibody (2C7) [NBP3-15189] - Immunofluorescence staining of HepG2 cells with at GSTO1 antibody 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189]

Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189] - IHC image of GSTO1 antibody diluted at 1:125 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.