Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GTF2A1 Antibodies »

Product Summary for GTF2A1 Antibody

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human Transcription Initiation Factor IIA Subunit 1 using the numbering given in entry NP_056943.1 (GeneID 2957).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTF2A1 Antibody

Immunohistochemistry: GTF2A1 Antibody [NBP1-46216]

Immunohistochemistry: GTF2A1 Antibody [NBP1-46216]

Immunohistochemistry: GTF2A1 Antibody [NBP1-46216] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- GTF2A1/TFIIA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: GTF2A1 Antibody [NBP1-46216]

Immunoprecipitation: GTF2A1 Antibody [NBP1-46216]

Immunoprecipitation: GTF2A1 Antibody [NBP1-46216] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-GTF2A1/TFIIA antibody used for IP at 3 mcg/mg lysate. GTF2A1/TFIIA was also immunoprecipitated by another rabbit anti-GTF2A1/TFIIA antibody, which recognizes an upstream epitope.

Applications for GTF2A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GTF2A1

GTF2A1 bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human GTF2A1 consists of three subunits of 35 kDa (alpha subunit), 19 kDa (beta subunit) and 12 kDa (gamma subunit). The alpha and beta units are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma subunit), retains native GTF2A1 activity.

Alternate Names

General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, MGC129969, MGC129970, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit

Gene Symbol

GTF2A1

Additional GTF2A1 Products

Product Documents for GTF2A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GTF2A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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